miR172 Can Move Long Distances in Nicotiana benthamiana
نویسندگان
چکیده
Small interfering RNAs can induce RNA silencing throughout plants owing to their ability to move through the phloem. Although microRNA (miRNA), another noncoding small RNA that regulates protein-coding RNAs through transcript cleavage or translational inhibition, has also been detected in phloem, there are few reports of its long-distance movement via phloem. We investigated whether miR172 molecules can move systemically from source tissues to sink tissues in Nicotiana benthamiana. Results of agro-infiltration and micro-grafting experiments indicate that miR172 can move long distances.
منابع مشابه
Systemic Spread of Sequence-Specific Transgene RNA Degradation in Plants Is Initiated by Localized Introduction of Ectopic Promoterless DNA
Systemic, posttranscriptional silencing of transgenes in Nicotiana benthamiana was initiated in localized regions of the plant by introduction of transgene-homologous DNA fragments, including those without a promoter. Following this initiation step, a sequence-specific signal of gene silencing spread from cells that had received the ectopic DNA via a relay mechanism that employs plasmodesmatal ...
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